摘要
目的:比较不同月龄大鼠阴茎海绵体组织中血红素氧合酶2(HO-2)及一氧化碳(CO)的含量,探讨HO-2/CO系统与年龄的相关性。方法:应用SABC免疫组化染色法观察8、16、24月龄(每组10只)大鼠阴茎组织中的HO-2并用图像分析法测定其含量;通过碳氧血红素曲线法测定阴茎组织中CO的含量。结果:阴茎海绵体组织中普遍存在HO-2,特别是在阴茎动脉外膜层周围及血窦内皮细胞系统中的含量较多,并且随着月龄的增长,阴茎海绵体组织中HO-2的含量逐步下降,高月龄组(16、24月龄)大鼠阴茎海绵体组织中HO-2含量明显低于低月龄组(8月龄)大鼠(P<0.05);阴茎海绵体组织中CO水平也随着大鼠月龄的增长显著降低(P<0.05)。结论:阴茎海绵体组织中因HO-2含量降低而导致CO含量下降且与年龄增长相关。
Objective： To compare the distribution of heme oxygenase-2 and concentration of carbon monoxide in rat penile tissue of 8, 16 and 24 months and investigate the relationship between the system of HO-2/CO and aging. Methods ： Using SABC immunohistoehemistry staining, image analysis system and the method of carboxyhemoglobin standard curve, the distribution of heme oxygenase-2 and concentration of carbon monoxide in different month rat penile tissues were investigated. Results ： The penile arteries were surrounded by HO-2 positive cells, which were also seen in the trabecular meshwork of smooth muscle. Compared with other part of penile, the penile arteries adventitia and the endothelial cells of cavernous exhibited darker staining. With the increasing of rat＇s living month the staining of penile tissues turned faint and the concentration of carbon monoxide in tissue decreased（ P 〈 0.05 ）. The imaging analysis system showed that the older the rat was the less HO-2 positive compositions contained （P 〈 0.05 ）. Conclusion ： With aging the decreasing concentration of HO-2 leads to the downfgt-regulation in rat penile tissue.
出处
《中华男科学杂志》
CAS
CSCD
2006年第5期424-427,共4页
National Journal of Andrology
