摘要
运用细胞原代培养技术、愈创木酚法及同位素法，观察不同浓度硝普钠（SNP）对甲状腺细胞甲状腺过氧化物酶（TPO）及碘有机化的影响。0、10^-6、10^-5、10^-4和10^-3mol／LSNP呈剂量依赖性抑制碘有机化[分别为（2708±250，2471±186，2319±159，l464±143，596±161）cpm／孔]。SNP（10^-6～10^-4mol／L）短时间（0．5h）孵育可增强甲状腺细胞TPO活性（0．027±0．004，0．033±0．002，0．050±0．003，0．040±0．003）U×10^-5，10^-3mol／LSNP对TPO活性没有影响（0．025±0．005）U×10^-5。不同浓度的SNP长时间（72h）孵育未显示对甲状腺细胞TPO活性有影响（P〉0．05）。碘有机化与TPO活性不相关。以上结果提示，NO抑制甲状腺细胞碘有机化，此作用可能与TPO无关。
Thyroid cells obtained from paraadenomatous tissue of thyroid adenomas were cultured in a monolayer system. The iodide organification was assayed in thyrocytes by radioactive isotope. Change in activity of thyroid peroxidase （TPO） was assayed by guaiacol, which is related to iodide organification. 0, 10^-6, 10^-5 , 10^-4 and 10^-3 mol/L nitroprusside （SNP） significantly inhibited the iodide organification in a dose-dependent mariner（2 708±250, 2 471±186, 2 319±159, 1 464±143, 596±216）cpm/well. When thyroeytes were incubated with 0 or 10^-6～10^-4 mol/L SNP for 0.5 h, TPO activities were enhanced [ （0. 027±0. 004; 0. 033 ±0.002; 0.050±0.003; 0.040±0. 003） U × 10^-5 ], but not under the concentration of 10^-3mol/L （0. 025±0.005 ） U × 10^-5. As thyroeytes were incubated with various concentrations of SNP for 72 h, TPO activity did not show statistical change. TPO activity was not related to iodide organification. The results suggested that NO inhibits iodide organification, and this action is probably not related to the activity of TPO in thyrocytes.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2006年第2期147-148,共2页
Chinese Journal of Endocrinology and Metabolism
