摘要
本文作者用化学发光测定法研究了丹参酮对趋化寡肽介导的人中性粒细胞活性氧产生的抑制效应。当分别用５、１０、２５、５０ｎｇ／ｍｌ丹参酮与中性粒细胞孵育后，再用ｆｍＬＰ刺激，发现各浓度的丹参酮均对其化学发光反应有显著抑制作用，抑制率分别为４５．２９％，６４．２３％，７５．２１％，８７．６４％。当用ＰＭＡ作为刺激剂时，亦发现丹参酮强烈抑制其介导的中性粒细胞化学发光反应。本实验结果提示丹参酮抑制中性粒细胞在炎症反应中产生氧自由基，可能是其抗炎症损伤的重要机制之一。
Tanshinone is a compound isolated from Chinese traditional herb medicine Tansnine.By using chemiluminescence assay we examined its inhibitory effect on the production of oxygen free radicals by human neutrophils.When preincubated with Tanshinone at 37℃ for 30 min and washed,chemiluminescence in neutrophils induced by fmLP was markedly deprossed.The depression rate was dose-dependent.When various concentrations of Tanshinone,e.g.5,10,25,and 50 ng/ml of the compound,were used,77.5%,81.7%,87.8% and 97.5% of depression respectively in neutrophil chemiluminescence reaction were observed.Alternatively,PMA,as a neutrophil stimulator,was also used in this assay system and similar results were observed.Because proteinkinase C has been known to be a receptor for PMA,this experiment suggested that Tanshinone may competitively inhibit proteinkinase C activation mediated by DAG on the pathway of chemotactic signal transduction.To confirm this presumption,further work on the cellular biochemistry analysis should be done.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1994年第6期635-638,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
