摘要
利用趋化方法测定了4-氯硝基苯(4-Chloronitrobenzene,4CNB)完全降解菌Pseudomonassp.ZWL73及其接合转移子ZP8对4CNB以及可能与4CNB降解相关的15种化合物的趋化能力.结果发现,两种菌株对4CNB等12种化合物均表现出趋化性,而这些化合物大多能被用作ZWL73的生长底物,这一结果显示了ZWL73对4CNB及其降解中间产物的趋化与降解转化之间的相关性.两种菌株对试验的化合物具有相同的趋化谱表明ZWL73对4CNB的趋化也是由4CNB降解质粒所控制.本研究首次报道了微生物对氯代硝基苯的趋化现象及其遗传学的初步研究.
Pseudomonas sp. strain ZWL73 grows on 4-chloronitrobenzene (4CNB) as a sole carbon and energy and its ability in degradation of 4CNB is plasmid-encoded. This strain and its plasmid transconjugant ZP8 (Psudomonas putida PaW340 harboring the 4CNB degradative plasmid) were tested in a chemotaxis assay for their ability to sense and respond to 4CNB and other 15 aromatic compounds that may be related to the 4CNB degradation. Both strains were found to be chemotactic to 12 compounds including 4CNB, most of which can also be used as growth substrates for strain ZWL73. The results obtained indicated a correlation between chemotaxis of strain ZWL73 towards the aromatics compounds tested and their degradation/transformation. The same chemotactic pattern towards these compounds between strain ZWL73 and its transconjugant ZP8 suggested the chemotaxis of ZWL73 to 4CNB was controlled by the very plasmid which codes for the function of 4CNB degradation. This is the first report on the chemotaxis of microorganisms to a chloronitrobenzene and a preliminary study on its genetics.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第6期624-627,共4页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金(30230010)
中国科学院百人计划和中国科学院创新工程资助项目.
