摘要
研究了大田期氮磷钾营养及配比对白肋烟中有关保护酶的影响及相关物质的动态。结果表明,过氧化物酶(POD)活性与脯氨酸(Pro)含量在烟株生长前期表现为上升趋势、后期呈现下降趋势,峰期出现在移栽后65d左右;多酚氧化酶(PPO)活性与总酚、类黄酮的含量变化呈现出随生育进程的推进而增加的单峰曲线,至移栽后75d达到高峰后回落;丙二醛(MDA)含量也随生育进程的推进而上升,总游离氨基酸(AA)含量则随生育进程的推进而逐渐下降。氮磷钾施用量与白肋烟内原保护酶活性及相关物质含量均呈正相关,N与总酚含量的相关性达极显著水平,N与POD、Pro、PPO和淀粉酶含量的相关性达显著水平。三元素对白肋烟中几种保护酶活性及相关物质含量的影响程度不完全相同,对PPO、Pro、总游离氨基酸和淀粉酶的影响程度为:N>P>K;对MDA、类黄酮含量的影响:K>N>P;对POD和总酚的影响:N>K>P。
The effects of N, P, K nutrients and their proportion on relative protective enzymes in burley tobacco and the dynamics of relative substances were studied in field stage. The results showed that the peroxidase (POD) activ-ity and proline (Pro) content went up in early growing stage and went down in late growing stage, the peak stage was at 65d or so after transplanting. The polyphenol oxidase (PPO) activity and contents of total phenol, flavonoid were increased as the advance of growing and developing, characterized by a curve with single peak, and the peak was at 75d after transplanting. The malondialdehyde (MDA) content went up and total free amino acid content went down as the process of growth. The application rate of N, P and K was positively correlated with endogenous protective enzyme activity and the contents of relative substances. The N showed an extremely significant correlation with total phenol and significant correlations with POD, Pro, PPO and amylase. The effects of the three elements on the activity of protective enzymes and contents of relative substances were different, the effects on PPO, Pro, total free amino acid and amylase were N > P > K, on the contents of MDA and flavonoid were K > N > P, and on POD and total phenol were N > K > P.
出处
《烟草科技》
EI
CAS
2004年第10期31-35,共5页
Tobacco Science & Technology
基金
中国烟草总公司和云南省烟草公司资助项目"云南优质白肋烟新品种选育及配套技术研究"(合同号99A03)的一部分
关键词
白肋烟
氮
磷
钾
营养
Burley tobacco
N
P
K
Nutrition
