摘要
丛枝菌根(AM)真菌多样性是当前菌根学领域研究热点之一。玫瑰是世界性花卉,在我国的栽培面积越来越大。为了解玫瑰根区土壤中AM真菌多样性与资源的分布状况,于2010年11月在济南平阴玫瑰园选择样地,采集了5个不同种/品种的蔷薇属(Rosa)植物根区土样,采用湿筛倾注——蔗糖离心法分离AM真菌,测定其物种丰度、频度、孢子密度、相对多度、重要值、多样性指数等。共分离到AM真菌4属28种,球囊霉属(Glomus)出现的频度最高,其次是无梗囊霉属(Acaulospora)。AM真菌孢子密度以‘紫枝’玫瑰(R.rugosa‘zizhi’)与山刺玫(R.davurica)根区的最高,野蔷薇(R.multiflora)最低;种丰度以‘丰花’(R.rugosa‘fenghua’)玫瑰最高;黄球囊霉(G.fulvum)是‘丰花’玫瑰的优势种,缩球囊霉(G.constrictum)是‘紫枝’玫瑰的优势种,网状球囊霉(G.reticulatum)是保加利亚白玫瑰(R.damascene)和山刺玫的优势种,双网无梗囊霉(A.bireticulata)是野蔷薇的优势种。认为玫瑰根区土壤中具有丰富的AM真菌物种多样性。本研究为进一步深入研究花卉植物根区AM真菌多样性提供了基础和依据。
Species diversity of arbuscular mycorrhizal(AM) fungi is one of research hots in mycorrhizology.Rose is a global flower,and its growing area increases year by year.In order to understand species diversity and resources distribution of AM fungi in root zone soil of rose plants,sampling sites were selected in Pingyin rosary in Jinan in November 2010,and the root zone soil sample of 5 different varieties of Rosa were collected to determine the diversity of arbuscular mycorrhizal fungi.Spores of AM fungi were identiffed to reveal the species richness,frequency,spore density,relative abundance,importance value and Shannon-Wiener indices of AM fungi.A total of 28 species belonging to 4 genera were identified according to the morphological characteristies of the spores isolated from soil samples collected using wet-sieving techniques.The relative abundance of the genus Glomus was the highest,followed by Acaulospora.Soil samples from 'Zizhi' and Rosa davurica showed the highest spore density,while R.multiflora was the lowest.Species richness in soil samples from 'Fenghua' was significantly higher than that in other varieties.G.fulvum was the dominant species of 'Fenghua',G.constrictum was the dominant species of 'Zizhi',G.reticulatum was the dominant species of 'Fenghua',R.damascene and R.davurica,Acaulospora bireticulata was the dominant species of R.multiflora.It is suggested that the species diversity in the root zone soil of rose plant be rich.The present research results provide a basis and foundation for further studying AM fungal diversity of flower plants.
出处
《青岛农业大学学报（自然科学版）》
2012年第3期157-163,共7页
Journal of Qingdao Agricultural University(Natural Science)
基金
国家自然科学基金资助项目(No.30871737)
青岛市自然科学基金资助项目(No.08-1-3-20-jch
12-1-4-5-(4)-jch)
关键词
菌根真菌
玫瑰园
丰度
频度
孢子密度
多样性
mycorrhizal fungi
rosary
species richness
frequency
spore density
diverstiy
