摘要
用光谱和电化学方法研究了儿茶酚胺衍生物与小牛胸腺 DNA之间的作用机制 .结果表明 ,低浓度的多巴酚丁胺和肾上腺素与 DNA之间主要为静电作用 ;而在高浓度时 ,则主要为插入作用 .对于多巴胺 ,在 5 .0 0×1 0 - 5～ 9.0 0× 1 0 - 4mol/L范围内 ,它与 DNA之间主要为插入作用 .同时 ,采用电化学方法测得多巴胺、肾上腺素和多巴酚丁胺与 DNA之间的表观结合常数分别为 1 .5 5× 1 0 3,9.77× 1 0 3和 1 .74× 1 0 4
The interactions between catecholamine derivatives and DNA were investigated by means of cyclic voltammetry, UV-Vis absorption spectra and fluorescence spectra. The results show that the interaction mode is mainly electrostatic interaction at the low concentration of dobutamine and adrenaline whereas intercalative binding plays a dominant role at their high concentration. As for dopamine, it intercalates into the double helix of DNA in the concentration range of 5 00×10 -5 to 9 00×10 -4 mol/L. The binding constants of dopamine, adrenaline and dobuamine with DNA are determined as 1 55×10 3, 9 77×10 3 and 1 74×10 4 L/mol, respectively, by electrochemical method.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第7期1235-1239,MJ02,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :90 2 0 60 3 7
2 0 2 0 5 0 0 7
2 0 0 75 0 10 )资助
