摘要
在云南发现的柑橘衰退病毒柠檬分离物对云南省德宏州的柠檬产业造成较大危害.通过用NdeⅠ和XhoⅠ对柑橘衰退病毒柠檬分离物外壳蛋白基因进行双酶切,定向插入到表达载体pET-28a中,并转化大肠杆菌BL21,经IPTG在28℃下诱导6 h后,SDS-PAGE电泳显示,在25 kDa处有特异表达谱带,回收特异蛋白带作为抗原免疫家兔,制备出特异抗血清.间接ELISA测得效价为1∶3000,并且与CTV具有良好的特异性反应.
The coat protein gene of citrus lemon isolate was cloned into expression vector pET-28a,and transferred into E.coli BL21.When induced with IPTG at 28 ℃ for 6h,it can be expressed as fusion protein which molecular weight is 25 kDa.This fusion protein was used as antigen to immue the rabbit and the specificity antibody was obtained.ID-ELISA showed that the antibody had titer of 1:3000 with high specificity to disease plant.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第S1期77-80,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省科技攻关计划(农业与社会发展部份)资助(2005NG02)
