摘要
目的研究烧伤并发抑郁症对学习记忆能力的影响。方法将清洁级成年雄性Wistar大鼠随机分为单纯烧伤组(30%深Ⅱ度烫伤)、单纯抑郁组、烧伤并发抑郁组、正常对照组。模型制造前,采用旷场实验筛选出符合条件的46只大鼠;在模型制造完毕后(第22天)对所有动物进行旷场行为、水迷宫、穿梭箱行为的实验。结果模型制造前采用旷场实验选择动物,各组大鼠旷场行为间差异均无统计学意义(P>0.05)。模型制造后,与正常对照组比较,抑郁组和烧伤并发抑郁组水平穿越格数、直立次数较少,差异均有统计学意义(P<0.05);烧伤组、抑郁组和烧伤并发抑郁组平均逃避潜伏期均长于正常对照组,且烧伤并发抑郁组最长(P<0.05);烧伤组、抑郁组和烧伤并发抑郁组对电刺激的主动和总回避率均低于正常对照组。结论通过对烧伤后大鼠慢性应激刺激,成功地诱导大鼠行为改变,显示出与临床烧伤后并发抑郁症相似的行为异常临床症状。烧伤后并发抑郁大鼠活动度减少,对外界缺乏兴趣,环境适应能力下降,反应降低,学习记忆力减弱。
Objective To observe the effects of chronic unpredicted mild stress after burn on the spatial learning and memory ability. Methods 46 male Wistar rats were divided randomly into 4 groups: the burn group, depression group, depression after burn and control group. Observed the open-field, water maze, shuttle back and forth box action after the models were developed. Results Before model making, no difference was seen among the rats in the open-field test (P>0.05). After the model developed, compared with the normal control group, the horizon and erect actions of the depression group and the depression after burn group decreased (P<0.05). The average latency of water maze and the initiative,passive and total avoidance rate of the burn group, depression group and depression after burn group were not as good as the normal control group(P<0.05). Conclusion The rat model of depression after burn has ecological validity, it mimics many of the symptoms of depression after burn patients. It displays the long-lasting significant abnormalities of emotional behaviors, such as behavioral habits, interesting last, maladapted to the surrounding, decrease on reaction.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第11期869-872,共4页
Journal of Environment and Health
关键词
烧伤
应激
行为障碍
学习
记忆
Burns
Stress
Conduct disorder
Learning
Memory
