摘要
目的研究河北省肉鸡养殖场分离的大肠埃希菌对常见抗菌药物的耐药情况。方法2017年3月,在河北省某2处大型肉鸡养殖场中选择38日龄的待宰肉鸡作为采样对象。将鸡舍分为5个区域采用单纯随机方法进行肉鸡泄殖腔拭子样品的采集,共得到样本273份。采用选择性培养基进行菌株分离,用VITEK~?-2型全自动微生物分析仪进行大肠埃希菌的鉴定和对10类18种常见抗菌药物的敏感性测试,并同时进行产超广谱β内酰胺酶(ESBL)的检测。结果经分离共得到菌落形态及生化特性不同的非重复的大肠埃希菌527株,产ESBL菌株占90.13%(475/527),对碳青霉烯类的厄他培南、美罗培南和亚胺培南的敏感率均为99.43%,对阿莫西林的耐药率最高,为99.24%。对四环素、环丙沙星、哌拉西林、氨曲南、左氧氟沙星的耐药率分别为94.88%、78.94%、78.37%、77.80%、70.40%。527株大肠埃希菌的多重耐药率为98.10%,且多重耐药菌株均产ESBL。耐受6类抗菌药物的菌株最多,占35.86%(189/527)。经分析共得到58种耐药谱,耐受青霉素类-头孢菌素类-氨曲南-氨基糖苷类-氟喹诺酮类-四环素-复方新诺明的菌株比重最高,占15.40%。结论肉鸡源大肠埃希菌对常见抗菌药物的耐药程度较高,多重耐药情况普遍存在,应加强对动物源菌株耐药性的监测和合理的轮换用药策略。
Objective To investigate and analyze the antibiotic resistance characteristics of Escherichia coli isolates from broilers.Methods The pre slaughter broilers were selected from 2 large poultry farms in Hebei province in March,2017.A total of 273 of cloacal swabs of the poultry from five different parts of the henhouses were collected.Selective agar was chosen for E.coli preliminary isolation,and VITEK~?-2 Compact 60 was used for isolate identification and antibiotic susceptibility test.Extended-spectrum beta-lactamase(ESBL)producing characteristics of the isolates were analyzed simultaneously.Results A total of 527 non-repetitive E.coli isolates were identified,in which 475(90.13%)isolates were ESBL-producing.The E.coli isolates showed the highest carbapenem-sensitivity(99.43%),including ertapenem,meropenem and imipenem,and the highest amoxicillin-resistance(99.24%),followed by tetracycline(94.88%),ciprofloxacin(78.94%),piperacillin(78.37%),aztreonam(77.80%),and levofloxacin(70.40%).All of the E.coli isolates were resistant to at least one group of antimicrobial agents,except for one isolate.Multidrug resistance was detected in the tested E.coli isolates(98.10%).All of the multidrug resistant E.coli isolates were ESBL producing.The most prevalent multidrug resistant isolates were those resistant to 6 groups of antimicrobial agents(35.86%,189/527).A total of 58 multidrug resistance spectrums were obtained.The proportion of the strains with multidrug resistance to penicillins,cephalosporins,aztreonam,aminoglycosides,flouroquinolones,tetracycline,sulfamethoxazole and trimethoprim was highest(15.40%)in antibiotic resistant E.coli isolates.Conclusion High prevalence of drug resistance and severe multidrug resistance were found in the tested E.coli isolates from broilers in Hebei.The multidrug resistance spectrums were complicated.It is necessary to strengthen the surveillance for E.coli of animal origin and continue the rational use of antibiotics.
作者
陈霞
赵晓菲
车洁
张云飞
袁敏
白雪梅
李娟
Chen Xia;Zhao Xiaofei;Che Jie;Zhang Yunfei;Yuan Min;Bai Xuemei;Li Juan(State Key Laboratory for Communicable Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《疾病监测》
CAS
2020年第1期46-51,共6页
Disease Surveillance
基金
国家自然科学基金(No.31502124,No.81861138053).
关键词
肉鸡
大肠埃希菌
耐药性
多重耐药性
Broiler
Escherichia coli
Antibiotic resistance
Multidrug resistance
