摘要
目的探究泛素结合酶E2Q1(Ubiquitin-conjugating enzyme E2Q1,UBE2Q1)的表达与肺腺癌临床病理特征及预后的相关性。方法回顾性了选取了2013年1月-2016年12月南京市胸科医院接受根治性手术治疗的74例I~Ⅲ期肺腺癌患者的癌组织及癌旁组织标本,采用免疫组化染色检测UBE2Q1蛋白表达水平,根据染色评分,将患者分为高表达组和低表达组,用卡方检验分析UBE2Q1蛋白水平与临床病理特征的相关性,KaplanMeier生存曲线分析UBE2Q1与肺腺癌患者预后相关性,单因素和多因素Cox比例风险模型分析影响肺腺癌患者生存期的危险因素。结果UBE2Q1在肺腺癌组织中高表达,且表达水平与肿瘤直径、淋巴结转移和TNM分期相关(P<0.05),与患者性别、年龄、吸烟史、肿瘤分化程度无相关性(P>0.05)。Kaplan-Meier生存分析结果显示,UBE2Q1低表达较UBE2Q1高表达患者有更长的无病生存期(disease-free survival,DFS)和总生存时间(overall survival,OS)(P均<0.05)。Cox比例风险模型显示肿瘤直径、淋巴结转移、TNM分期以及UBE2Q1高表达是DFS、OS的危险因素,其中TNM分期是DFS、OS的独立危险因素。结论UBE2Q1在肺腺癌组织中高表达,且与肺腺癌肿瘤直径大、淋巴结转移、TNM分期晚及较差预后相关,UBE2Q1是肺腺癌的危险因素。
Objective To investigate the correlation between UBE2Q1 expression and clinicopathologic features and prognosis of lung adenocarcinoma.Methods This study retrospectively chose the cancer tissue and para-carcinoma tissue samples of 74 patients with stage I to III lung adenocarcinoma who received radical resection in Nanjing Chest Hospital from January 2013 to December 2016.Immunohistochemistry staining was used to detect the expression level of UBE2Q1,and patients were divided into high-expression group and low-expression group according to the Immunohistochemistry staining score.The correlation of UBE2Q1 expression level and clinicopathological characteristics was analyzed by Chi-square test.Kaplan-Meier survival curve analyzed the correlation between UBE2Q1 and prognosis of lung adenocarcinoma patients.The risk factors affecting the survival of lung adenocarcinoma patients were analyzed by univariate and multivariate Cox proportional risk models.Results UBE2Q1 was highly expressed in lung adenocarcinoma tissues,and the expression level was correlated with tumor diameter,lymph node metastasis,and TNM stage(P<0.05),and did not correlate with patients’gender,age,smoking history,and tumor differentiation(P>0.05).The results of the Kaplan-Meier survival analysis showed that patients with low expression of UBE2Q1 compared with those with high expression of UBE2Q1 had longer DFS and OS(both P<0.05).Cox proportional risk model showed that tumor diameter,lymph node metastasis,TNM stage,and high UBE2Q1 expression were the risk factors for DFS and OS,among which TNM stage was an independent risk factor.Conclusion UBE2Q1 was highly expressed in lung adenocarcinoma tissues and correlated with large tumor diameter,lymph node metastasis,late TNM stage and poorer prognosis in lung adenocarcinoma,and UBE2Q1 was a risk factor for lung adenocarcinoma.
作者
蒋微
王羽超
徐春华
邹珏
张倩
JIANG Wei;WANG Yuchao;XU Chunhua;ZOU Jue;ZHANG Qian(Department of Respiratory Medicine,Nanjing Chest Hospital,Nanjing,Jiangsu 210029,P.R.China;Respiratory Medicine Department of Thoracic Hospital,the Affiliated Nanjing Brain Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,P.R.China;Department of Pathology,the Affiliated Nanjing Brain Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,P.R.China)
出处
《中国呼吸与危重监护杂志》
CAS
CSCD
2024年第4期237-241,共5页
Chinese Journal of Respiratory and Critical Care Medicine
基金
南京市医学科技发展重点项目(ZKX17044)
